## Slide #1.

Recall that our first case of thinking about pigments in the eye was to consider rhodopsin alone (one pigment) and the implications of its absorption spectrum. We had two lights, each with one wavelength. The left hand light was fixed and the right hand light could be varied in intensity. Could there be any intensity where the two lights look identical even though their wavelengths are different? Now add a pigment to the receptor system. Compare the response of two pigments to the two lights, each with a different wavelength from the other (one wavelength).
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## Slide #2.

• Case 1 -- Equal number of photons at each of the two different wavelengths
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## Slide #3.

wo different wavelength lights indicated by Greek lambdas Incident Photons 1000   Pigment 1 Pigment 2 Photons Absorbed Photons Absorbed 460 150 Incident 1000 Pigment 1 260 Pigment 2 410
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## Slide #4.

• Case 2 -- Turn up the intensity of the adjustable light on the right. What happens to the number of photons absorbed by pigment 1 and pigment 2? Does either pigment match the corresponding number for the left hand light? • Remember: Once the wavelengths have been selected and the observer with a certain number of visual pigments has been selected, only the intensity (amount, number of photons) of the lights can be changed in the experiment.
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## Slide #5.

Incident Photons 1000 Pigment 1 Pigment 2 Photons Absorbed Photons Absorbed 460 150 Incident 1770 Pigment 1 Pigment 2 Photons Absorbed 460 725
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## Slide #6.

• Case 3 -- Turn down the intensity of the adjustable light on the right. What happens to the number of photons absorbed by pigment 1 and pigment 2? Does either pigment match the corresponding number for the left hand light?
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## Slide #7.

Incident Photons 1000 Pigment 1 Pigment 2 Photons Absorbed Photons Absorbed 460 150 Incident 366 Pigment 1 95 Pigment 2 150
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## Slide #8.

The two lights NEVER match
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## Slide #9.

Summary Table • What have we learned? As the wavelength intensity is adjusted, is there any place where the two lights exactly match? Yes, if we have one underlying pigment. No if we have 2 underlying pigments Number of wavelengths in the adjustable light 1 1 Number of underlying pigments 2 3 Y N 2 3 4 5 6
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## Slide #10.

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## Slide #11.

Spectral absorption curves for the two hypothetical pigments and two adjustable wavelengths in the light on the right (2 and 3 ) compared to the fixed light on the left (1)
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## Slide #12.

Pigment 1 Pigment 2 Wavelengths in Lights
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## Slide #13.

Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. Set-up: Light with one wavelength compared to light with two wavelengths.  ye  Person whose eye has two cone types (pigments) Blow-up diagram of small portion of retina – suppose there are two cone types, meaning 2 pigments
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## Slide #14.

 Incident Photons 1 1000 2 0 3 0  Pigment 1 Pigment 2 Photons Absorbed Photons Absorbed 530 260 0 0 -------Total Effect 530 0 0 ----------260 Incident Pigment 1 Pigment 2 0 0 0 1000 360 110 1000 270 ----------630 410 ----------520
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## Slide #15.

Incident Photons 1 1000 2 0 3 0 Pigment 1 Pigment 2 Photons Absorbed Photons Absorbed 530 260 0 0 -------Total Effect 530 0 0 ----------260 Incident Pigment 1 Pigment 2 0 0 0 1250 450 137 300 80 ----------530 123 ----------260
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## Slide #16.

Now you got the two lights to match perfectly again. To do this you had to have two adjustable wavelengths being shined on two pigments
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## Slide #17.

Summary Table As the wavelength intensity is adjusted, is there any place where the two lights exactly match? Number of wavelengths in the adjustable light 1 Number of underlying pigments 2 3 1 2 Y N Y 3 4 5 6
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## Slide #18.

Now add a 3rd underlying pigment What will happen when we try to match the light on the right, with two adjustable pigments, to the light on the left when there are 3 underlying responding pigments?
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## Slide #19.

Pigment 1 Pigment 2 Draw a third curve in here Wavelengths in Lights
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## Slide #20.

Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. Set-up: Light with one wavelength compared to light with two wavelengths.  ye  Person whose eye has three cone types (pigments) Blow-up diagram of small portion of retina – suppose there are three cone types, meaning 3 pigments
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## Slide #21.

No Match
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## Slide #22.

Summary Table As the wavelength intensity is adjusted, is there any place where the two lights exactly match? Number of wavelengths in the adjustable light 1 1 Number of underlying pigments 2 2 Y Y N 3 3 4 5 6
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## Slide #23.

Now add a 3rd adjustable wavelength to the light on the right
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## Slide #24.

Matching task: Can we adjust the amount of light in each light so that the lights look identical? If “yes,” then wavelength and intensity (amount of light) cannot be distinguished. If “no,” then wavelength and intensity can be distinguished. Set-up: Light with one wavelength compared to light with three wavelengths.  ye  Person whose eye has two cone types (pigments) Blow-up diagram of small portion of retina – suppose there are three cone types, meaning 3 pigments
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## Slide #25.

Summary Table As the wavelength intensity is adjusted, is there any place where the two lights exactly match? Number of wavelengths in the adjustable light 1 1 Number of underlying pigments 2 2 Y N Y N 3 3 Y 4 5 6
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## Slide #26.

Now add a 4th underlying pigment. Will you ever get a match between the lights if there are 3 intensity adjustable wavelengths added together in the light on the right?
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## Slide #27.

What would the table look like if we kept filling it in?
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